It was very hard to insert the small 5cm 3 beaker into the conical flask, and when it came to tipping it over, some of the substrate was still trapped inside the beaker. In human body, most enzyme work fastest at an optimum pH of about 7. The decomposition reaction of hydrogen peroxide can be boosted by injection of higher concentration of catalase into animal or plant cells if it is determined in this experiment that higher concentration of catalase yields higher rate of decomposition of hydrogen peroxide. Without catalase, the decomposition would take much longer and would not be fast enough to sustain human life. All the reactions must be carried out for a standard amount of time.

Lab instructor grinded potatoes in a blender and filtered out the solid residue. The hydrogen peroxide is injected into the test tube, and the volume of oxygen gas is recorded by the amount of water displaced , determining the rate of reaction. Apparatus Limitations Firstly, there were limitations on the apparatus that I used. However, the inhibitors are not permanently bound to the active site and so, when it leaves another molecule can take its place. I have drawn a line of best fit to clearly illustrate this trend.

Despite their large overall size, enzyme molecules only have a small region that is functional.

More collisions are therefore successful, so more substrate is converted into product. The height of foam in each test tubes were recorded in google document. Catalase binds with the substrate molecules though their active sites courseeork catalyses by processing them to form the ultimate by-products of water and oxygen, at a rapid rate as it has high turnover numbers.

At low temperature, the reaction takes place very slowly, this because molecules are moving relatively slowly as have low kinetic energy. This is feature allows you to search the site.


Effect of Substrate Concentration on the Rate of Activity of Catalase

Recording Results I will record my results in a table like the one below, and then record further, average results, in a similar table. This service allows you to sign up for or associate a Google AdSense account with HubPages, vatalase that you can earn money from ads on your articles.

I’m doing my GCSE additional science coursework. This happened due to lack of trials. This is called the maximum velocity of the reaction or Vmax. This is an ad network.

catalase hydrogen peroxide coursework

Represent the results in a graph in order to work out the gradient and make a conclusion based on the evidence you have obtained. Above the optimum temperature, the thermal energy breaks the hydrogen bonds holding the secondary and tertiary structure together, so the active site changes shape and eventually the reaction can no longer be catalysed.

According to the Michaelis-Menton mechanism, there are three principal features of many enzyme catalysed reactions and they are as follows: The effect of concentration of catalase on the rate of decomposition of hydrogen peroxide. The balance proved to be the biggest apparatus error and this would have been much bigger if I had used only 0.

This is a simple reaction and proceeds as follows: This structure is known as enzyme-substrate complex. Because the volume of oxygen dissolved in the reaction is not constant for all reactions, and less oxygen is dissolved in water at higher temperatures, this would have affected my results.

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Size Gcse Coursework Biology Potato Catalase Hydrogen Peroxide

The result supports the hypothesis in this experiment. So, although my results did show a positive correlation, it wasn’t necessarily an accurate correlation because my results do not follow specific trends.

catalase hydrogen peroxide coursework

Limitations are individual activity within the classroom which can change the temperature and pressure in the room. Couesework addition, based on my knowledge of the collision theory I believe that if the concentration of hydrogen peroxide is doubled or halved then the rate of reaction is also doubled or halved.


Size Gcse Coursework Biology Potato Catalase Hydrogen Peroxide

Catakase a temperature below the optimum, the molecules have less kinetic energy, so the rate of collisions between enzyme and substrate molecules is low, therefore less enzyme-substrate complexes are formed. We may use remarketing pixels from advertising networks such as Google AdWords, Bing Ads, and Vatalase in order to advertise the HubPages Service to people that have visited our sites. Each piece of apparatus has an apparatus error with an upper and lower limit. In this case, I will use coyrsework peroxide cousrework the substrate.

Ib Biology Coursework Catalase – relojesdesilicon. Apparatus Error Apparatus error was one of the main factors in my experiment which I tried to keep at a minimum. Temperature directly affects the shape of the active site.

Substrate molecules will not often collide with the active site, and so binding between substrate and enzyme is a rare event. Leave your email and we will send you an example after 24 hours Concentration of Hydrogen Peroxide The different concentrations of hydrogen peroxide that I made could not have been exactly accurate because this would have meant that the volume of gas evolved would have increased in equal steps, which it did not.